IBMX
小眼畸形相关转录因子
化学
酪氨酸酶
黑色素
皮肤色素沉着
MAPK/ERK通路
皂甙
生物化学
药理学
细胞毒性
色素沉着
生物
福斯科林
信号转导
酶
体外
免疫毒素
作者
Jung‐Soo Bae,Mira Han,Yao Cheng,Jin Ho Chung
标识
DOI:10.1016/j.cbi.2015.12.021
摘要
Chaetocin is a natural product isolated from Chaetomium species that has anti-bacterial and anti-myeloma activities. In this study, we investigated the inhibitory effect of chaetocin on melanogenesis and the underlying mechanisms in B16F10 mouse melanoma cells. In the present study, chaetocin significantly inhibited IBMX-induced melanin production and tyrosinase activity without any cytotoxicity. Furthermore, chaetocin down-regulated both the protein and mRNA levels of tyrosinase, which is a specific enzyme that catalyzes the conversion of tyrosine to melanin. We also observed that the protein level of MITF was significantly reduced by chaetocin treatment. In addition, we found that the anti-melanogenic effect of chaetocin was suppressed by treatment with the specific ERK inhibitor (PD98059). Accordingly, chaetocin inhibited melanogenesis via suppressing the protein level of MITF followed by activation of the ERK signaling pathway. These data suggest that chaetocin may be a potential anti-melanogenic agent for use in skin-whitening cosmetics and a topical agent for treatment of hyperpigmentation disorders.
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