干细胞
生物发光成像
间充质干细胞
旁分泌信号
移植
细胞外基质
干细胞疗法
血管生成
医学
壳聚糖
癌症研究
自愈水凝胶
活力测定
细胞疗法
化学
细胞生物学
细胞
生物
内科学
生物化学
转染
受体
荧光素酶
有机化学
基因
作者
Guowei Feng,Jimin Zhang,Li Yang,Yan Nie,Dashuai Zhu,Ran Wang,Jianfeng Liu,Jie Gao,Na Liu,Ningning He,Wei Du,Hongyan Tao,Yongzhe Che,Yong Xu,Deling Kong,Qiang Zhao,Zongjin Li
出处
期刊:Journal of The American Society of Nephrology
日期:2016-02-11
卷期号:27 (8): 2357-2369
被引量:107
标识
DOI:10.1681/asn.2015050578
摘要
Low cell retention and engraftment after transplantation limit the successful application of stem cell therapy for AKI. Engineered microenvironments consisting of a hydrogel matrix and growth factors have been increasingly successful in controlling stem cell fate by mimicking native stem cell niche components. Here, we synthesized a bioactive hydrogel by immobilizing the C domain peptide of IGF-1 (IGF-1C) on chitosan, and we hypothesized that this hydrogel could provide a favorable niche for adipose-derived mesenchymal stem cells (ADSCs) and thereby enhance cell survival in an AKI model. In vitro studies demonstrated that compared with no hydrogel or chitosan hydrogel only, the chitosan–IGF-1C hydrogel increased cell viability through paracrine effects. In vivo , cotransplantation of the chitosan–IGF-1C hydrogel and ADSCs in ischemic kidneys ameliorated renal function, likely by the observed promotion of stem cell survival and angiogenesis, as visualized by bioluminescence imaging and attenuation of fibrosis. In conclusion, IGF-1C immobilized on a chitosan hydrogel provides an artificial microenvironment for ADSCs and may be a promising therapeutic approach for AKI.
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