Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications

YeastVolume 14, Issue 2 p. 115-132 Research Article Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications Carrie Baker Brachmann, Carrie Baker Brachmann Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorAdrian Davies, Adrian Davies Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorGregory J. Cost, Gregory J. Cost Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorEmerita Caputo, Emerita Caputo Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorJoachim Li, Joachim Li Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorPhilip Hieter, Philip Hieter Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorJef D. Boeke, Corresponding Author Jef D. Boeke jef.boeke@qmail.bs.jhu.edu. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Hunterian 617, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, U.S.A. Tel: (+1) 410 955 2481; fax: (+1) 410 614 2987Search for more papers by this author Carrie Baker Brachmann, Carrie Baker Brachmann Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorAdrian Davies, Adrian Davies Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorGregory J. Cost, Gregory J. Cost Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorEmerita Caputo, Emerita Caputo Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorJoachim Li, Joachim Li Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorPhilip Hieter, Philip Hieter Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Search for more papers by this authorJef D. Boeke, Corresponding Author Jef D. Boeke jef.boeke@qmail.bs.jhu.edu. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.Hunterian 617, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, U.S.A. Tel: (+1) 410 955 2481; fax: (+1) 410 614 2987Search for more papers by this author First published: 04 December 1998 https://doi.org/10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2Citations: 204AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. © 1998 John Wiley & Sons, Ltd. Citing Literature Volume14, Issue230 January 1998Pages 115-132 RelatedInformation