纳米尺度
肺表面活性物质
生物物理学
纳米技术
染色体易位
折叠(高阶函数)
DNA
材料科学
化学
分子动力学
化学工程
结晶学
生物
计算化学
机械工程
基因
生物化学
工程类
作者
Neeraj Soni,Navneet C. Verma,Noam Talor,A. Meller
出处
期刊:Nano Letters
[American Chemical Society]
日期:2023-05-07
卷期号:23 (10): 4609-4616
被引量:6
标识
DOI:10.1021/acs.nanolett.3c01096
摘要
Solid-state nanopores (ssNPs) are single-molecule sensors capable of label-free quantification of different biomolecules, which have become highly versatile with the introduction of different surface treatments. By modulating the surface charges of the ssNP, the electro-osmotic flow (EOF) can be controlled in turn affecting the in-pore hydrodynamic forces. Herein, we demonstrate that negative charge surfactant coating to ssNPs generates EOF that slows-down DNA translocation speed by >30-fold, without deterioration of the NP noise, hence significantly improving its performances. Consequently, surfactant-coated ssNPs can be used to reliably sense short DNA fragments at high voltage bias. To shed light on the EOF phenomena inside planar ssNPs, we introduce visualization of the electrically neutral fluorescent molecule's flow, hence decoupling the electrophoretic from EOF forces. Finite elements simulations are then used to show that EOF is likely responsible for in-pore drag and size-selective capture rate. This study broadens ssNPs use for multianalyte sensing in a single device.
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