微尺度化学
微型反应器
纳米技术
微流控
辣根过氧化物酶
混合(物理)
微观混合
化学
磁场
光热治疗
放热反应
材料科学
催化作用
有机化学
酶
数学教育
物理
量子力学
数学
作者
Yuting Liao,Dong Liu,Bin Wang,Zijian Wu,Miao Ni,Mengchen Wang,Chenghua Wang,Yuan Lu
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2022-10-20
卷期号:6 (1): 21-33
被引量:6
标识
DOI:10.1021/acsanm.2c03295
摘要
The intensification of microreactions is important for biological and chemical reactions with microliter volumes as reaction units, including microliter bioassay or protein synthesis. At the micro/nanoscale, the fluid flow is usually located in the laminar region, making the interaction between different layers of material more difficult. In the absence of external intervention, the microscale transport of substances is dominated by passive diffusion, leading to a lower mixing efficiency and longer reaction times, which is disadvantageous for reactions requiring timeliness, such as microliter bioassays. Therefore, the intensification of microreactions is essential. Here, a dual magnetic–photothermal microreaction control platform was created, based on the cooperative roles of a close-loop photothermally controlled magnetically active nanoswarm (PMANS) as a nanostir for quick fluid mixing and as a nanoheating agent for the reaction solution. The platform was demonstrated to be enhanced for microreactions by using the glucose oxidase and horseradish peroxidase cascade and cell-free synthetic superfolder green fluorescent protein reactions, which are typical in the fields of diagnostic testing and biomanufacturing, respectively. The rotational motion of the PMANS is controlled by the magnetic field to achieve efficient and homogeneous mixing of fluids at the microscale. On the other hand, the PMANS is used to heat the reaction system through light control. The construction of a dual magnetic–temperature control platform provided a new strategy for microreaction enhancement that was convenient, controllable, and broadly applicable. This control platform has great potential for microscale reaction enhancement in detection, sensing, catalysis, and synthesis.
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