少突胶质细胞
奥利格2
生物
髓鞘
髓鞘碱性蛋白
免疫染色
谱系标记
蛋白脂蛋白1
转基因小鼠
细胞生物学
病理
转基因
神经科学
祖细胞
免疫学
免疫组织化学
干细胞
中枢神经系统
遗传学
基因
医学
作者
Tomohiro Torii,Yuki Miyamoto,Rinaho Nakata,Yuto Higashi,Yohei Shinmyo,Hiroshi Kawasaki,Tomohiro Miyasaka,Hiroaki Misonou
出处
期刊:Glia
[Wiley]
日期:2022-12-24
卷期号:71 (4): 1002-1017
被引量:3
摘要
Abstract Microtubule‐associated protein Tau is primarily expressed in axons of neurons, but also in Olig2‐positive oligodendrocytes in adult rodent and monkey brains. In this study, we sought to determine at what cell stage Tau becomes expressed in the oligodendrocyte lineage. We performed immunostaining of adult mouse brain sections using well‐known markers of oligodendrocyte lineage and found that Tau is expressed in mature oligodendrocytes, but not in oligodendrocyte progenitors and immature pre‐oligodendrocytes. We also investigated Tau expression in developing mouse brain. Surprisingly, Tau expression occurred after the peak of myelination and even exceeded GSTπ expression, which has been considered as a marker of myelinating oligodendrocytes. These results suggest Tau as a novel marker of oligodendrocyte maturation. We then investigated whether Tau is important for oligodendrocyte development and/or myelination and how Tau changes in demyelination. First, we found no changes in myelination and oligodendrocyte markers in Tau knockout mice, suggesting that Tau is dispensable. Next, we analyzed the proteolipid protein 1 transgenic model of Pelizaeus‐Merzbacher disease, which is a rare leukodystrophy. In hemizygous transgenic mice, the number of Tau‐positive cells were significantly increased as compared with wild type mice. These cells were also positive for Olig2, CC1, and GSTπ, but not PDGFRα and GPR17. In stark contrast, the expression level of Tau, as well as GSTπ, was dramatically decreased in the cuprizone‐induced model of multiple sclerosis. Taken together, we propose Tau as a new marker of oligodendrocyte lineage and for investigating demyelination lesions.
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