High mobility group box 1 derived mainly from platelet microparticles exacerbates microvascular obstruction in no reflow

HMGB1 医学 促炎细胞因子 流式细胞术 经皮冠状动脉介入治疗 血小板活化 心脏病学 单核细胞 炎症 血小板 内科学 免疫学 心肌梗塞
作者
Xinyi Zhao,Jianbin Han,Lijin Zhou,Jinjin Zhao,Meijiao Huang,Yueqing Wang,Junjie Kou,Yan Kou,Jiaqi Jin
出处
期刊:Thrombosis Research [Elsevier BV]
卷期号:222: 49-62 被引量:6
标识
DOI:10.1016/j.thromres.2022.12.003
摘要

No reflow manifests coronary microvascular injury caused by continuous severe myocardial ischemia and reperfusion. Microvascular obstruction (MVO) has emerged as one fundamental mechanism of no reflow. However, the underlying pathophysiology remains incompletely defined. Herein, we explore the contribution of high mobility group box 1 (HMGB1), derived mainly from platelet microparticles exacerbating MVO in no reflow.44 STEMI patients undergoing successful primary percutaneous coronary intervention (PCI) were included in our study. Plasma HMGB1 levels in both the peripheral artery (PA) and infarct-related coronary artery (IRA) were measured by ELISA. Flow cytometry and confocal microscopy assessed the level of HMGB1+ platelet derived microparticles (PMPs) and platelet activation. Flow cytometry and western blot evaluated the procoagulant activity (PCA) and the release of inflammatory factors of human microvascular endothelial cells (HCEMCs).HMGB1 levels were significantly higher in the IRA in no-reflow patients. The levels of HMGB1+ PMPs were considerably higher in the IRA of patients with no reflow and were strongly associated with platelet activation. Moreover, our results show that HMGB1 interacts with human microvascular endothelial cells primarily through TLR4, inducing HCMEC proinflammatory, procoagulant phenotype, and monocyte recruitment, accelerating microvascular obstruction and facilitating the development of no reflow.Our results illustrate a novel mechanism by which HMGB1, derived mainly from PMPs, plays a crucial role in the pathogenesis of no-reflow, revealing a novel therapeutic target.
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