显微镜
荧光
近场和远场
光激活定位显微镜
荧光显微镜
分辨率(逻辑)
纳米技术
材料科学
光学显微镜
超分辨显微术
光学
光电子学
物理
扫描电子显微镜
计算机科学
人工智能
作者
Guangyong Qin,Xin Guan,Jian Mao,Zhenzhen Feng,Wenxuan Yang,Shenming Wang,Zhen Zheng,Xintong Miao,Yifeng Cheng,Zhirui Wang,Xiaojuan Wang,Fang Huang,Hua He
出处
期刊:Nano Letters
[American Chemical Society]
日期:2024-11-12
卷期号:24 (47): 15186-15194
被引量:1
标识
DOI:10.1021/acs.nanolett.4c05155
摘要
Fluctuation-based super-resolution microscopy enhances image resolution using signal fluctuations, yet the inherent fluctuations of fluorophores limit its spatiotemporal resolution. In this work, we reveal a far-field enhancement (FFE) effect via a nanosilver film that significantly boosts fluorescence fluctuations of fluorophores positioned up to 10 μm away. The FFE effect arises from the interference of scattered light from the nanosilver film and photothermal-induced refractive index changes in the imaging medium, which create periodic auxiliary illumination on the sample. This phenomenon enabled the development of far-field enhanced super-resolution microscopy (FFE-SRM), a technique compatible with commonly used fluorophores. FFE-SRM improves temporal resolution up to 10-fold and enhances spatial resolution by about 2-fold over various SRM methods, including stochastic optical fluctuation imaging, super-resolution radial fluctuation, mean-shift super-resolution, and direct stochastic optical reconstruction microscopy. We demonstrated the potential of FFE-SRM by revealing mitochondrial dynamics in live-cell imaging, advancing super-resolution imaging, and cellular process exploration.
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