脚手架
生物医学工程
体内
化学
组织工程
去卵巢大鼠
体外
基质(化学分析)
材料科学
细胞
细胞生物学
生物
医学
生物技术
激素
生物化学
色谱法
作者
YoungWon Koo,Hyeongjin Lee,Chang Su Lim,Su Yeon Kwon,Inbo Han,GeunHyung Kim
标识
DOI:10.1016/j.ijbiomac.2022.09.249
摘要
Efficient vascularization within a scaffold is an essential criterion for evaluating the success of volumetric bone formation. Various strategies using angiogenic growth factors and cell-based approaches to induce effective osteogenic and angiogenic activities have been investigated. In this study, we propose a new highly porous multiple-cell-laden collagen/hydroxyapatite scaffold fabricated using a whipped bioink. After in vitro culturing of cells in the porous scaffolds for an extended culture period, osteogenic and angiogenic activities were significantly enhanced owing to the well-developed microporous cell-supporting matrix inducing efficient crosstalk between the adipose stem cells and endothelial cells compared to those of the normally bioprinted cell-constructs. Furthermore, the in vitro results were thoroughly evaluated by in vivo experiments using a posterolateral lumbar spinal fusion model of an ovariectomized mouse. Based on these results, the porous multiple-cell-laden scaffolds enhanced spine fusion in the event of osteoporosis.
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