化学
检出限
适体
石墨烯
电极
组合化学
电化学
基质(水族馆)
亚甲蓝
分析化学(期刊)
纳米技术
色谱法
有机化学
物理化学
遗传学
材料科学
海洋学
光催化
地质学
生物
催化作用
作者
Jia Wu,Baoshan He,Yuling Wang,Renyong Zhao,Yurong Zhang,Chunqi Bai,Min Wei,Huali Jin,Wenjie Ren,Zhiguang Suo,Yiwei Xu
出处
期刊:Talanta
[Elsevier]
日期:2023-09-30
卷期号:267: 125257-125257
被引量:9
标识
DOI:10.1016/j.talanta.2023.125257
摘要
In this work, a novel ultrasensitive aptasensor for deoxynivalenol (DON) detection based on the polyethyleneimine-functionalised porous reduced graphene oxide loaded gold nanowires (PEI-PrGO/AuNWs) and methylene blue (MB)-labelled imidazole molecular sieve backbone (ZIF-8) signal amplification strategy was proposed. PEI-PrGO/AuNWs with large surface area and excellent conductivity were used as modification materials on bare gold electrodes, which could increase the combining of complementary strand (cDNA) on the electrode substrate and accelerate the electron transfer efficiency. Furthermore, a novel electrochemical signal probe was synthesized using streptavidin-modified imidazole molecular sieve backbone (ZIF-8/SA) as a carrier loaded with MB and reverse complementary chain (sDNA). In the presence of DON, the signal probe was introduced to the electrode surface by Watson-Crick base pairing after specific binding of DON to the aptamer (Apt). As expected, under the optimal conditions, the DON concentration was linearly related to the peak current generated by the prepared aptasensor, and the measured data were combined with theoretical calculations to obtain a detection limit of 2.23 × 10−9 mg/mL.
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