Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis

多杀性巴氏杆菌 毒力 生物 微生物学 生物膜 巴氏杆菌病 病菌 基因 吞噬作用 细菌 遗传学
作者
Qingjie Lv,Yuyao Shang,Haixin Bi,Jie Yang,Lin Lin,Congcong Shi,Mixue Wang,Rui Xie,Zhanwei Zhu,Fei Wang,Lin Hua,Huanchun Chen,Bin Wu,Zhong Peng
出处
期刊:Microbes and Infection [Elsevier]
卷期号:27 (1): 105235-105235 被引量:6
标识
DOI:10.1016/j.micinf.2023.105235
摘要

Two-component regulatory system (TCS) is a widespread bacterial signal transduction mechanism and plays a critical role in bacterial adaptation to environments as well as regulating bacterial virulence. However, few studies have reported the actions of TCS in Pasteurella multocida, a zoonotic bacterial pathogen. In this study, genes encoding proteins homologous to the ArcAB TCS were identified in genome sequences of P. multocida belonging to different serogroups, and the transcription of both arcA and arcB was up-regulated in anaerobic and superoxygen environment. Compared to wild type strains, P. multocida arcA-deletion mutants (ΔarcA) displayed a decrease in growing under anaerobic conditions, biofilm formation, as well as the capacities of anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in different in vivo models (Galleria mellonella and mice). RNA-Seq identified 70 significantly downregulated genes in ΔarcA compared to the wild type strain, and several of them are associated with P. multocida virulence. Among them, a universal stress protein E encoding gene uspE was characterized in P. multocida for the first time. Electrophoretic mobility shift assay (EMSA) demonstrated that the ArcAB TCS could regulate uspE directly. Deletion of uspE also led to a decrease of P. multocida in growing under anaerobic conditions, biofilm formation, anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in mice. The data provided from this study will help further understanding the fitness and pathogenesis of P. multocida.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
YIN完成签到 ,获得积分10
刚刚
刚刚
我的光完成签到,获得积分10
刚刚
大个应助pierniao采纳,获得10
1秒前
1秒前
1秒前
2秒前
南小雪发布了新的文献求助10
2秒前
干净的琦应助391X小king采纳,获得10
2秒前
3秒前
林一发布了新的文献求助10
3秒前
stewie发布了新的文献求助30
3秒前
慕青应助wzwz采纳,获得10
3秒前
3秒前
3秒前
4秒前
于思枫发布了新的文献求助10
4秒前
lxaiczn发布了新的文献求助10
5秒前
杙北完成签到 ,获得积分10
5秒前
跳跳狗发布了新的文献求助10
5秒前
乐可乐发布了新的文献求助10
5秒前
豆沙包完成签到 ,获得积分10
5秒前
CJW发布了新的文献求助10
6秒前
6秒前
可爱的函函应助糖小白采纳,获得10
6秒前
kommon完成签到,获得积分20
7秒前
7秒前
7秒前
8秒前
8秒前
愉快幻悲发布了新的文献求助10
8秒前
8秒前
黎JX发布了新的文献求助10
8秒前
精明冰巧发布了新的文献求助10
10秒前
10秒前
李子完成签到,获得积分10
10秒前
程嘉玲完成签到,获得积分10
10秒前
11秒前
11秒前
11秒前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Modern Epidemiology, Fourth Edition 5000
Kinesiophobia : a new view of chronic pain behavior 5000
Molecular Biology of Cancer: Mechanisms, Targets, and Therapeutics 3000
Digital Twins of Advanced Materials Processing 2000
Weaponeering, Fourth Edition – Two Volume SET 2000
Signals, Systems, and Signal Processing 610
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 纳米技术 化学工程 生物化学 物理 计算机科学 内科学 复合材料 催化作用 物理化学 光电子学 电极 冶金 细胞生物学 基因
热门帖子
关注 科研通微信公众号,转发送积分 6016504
求助须知:如何正确求助?哪些是违规求助? 7598486
关于积分的说明 16152466
捐赠科研通 5164217
什么是DOI,文献DOI怎么找? 2764624
邀请新用户注册赠送积分活动 1745571
关于科研通互助平台的介绍 1634954