乙酸乙酯
抗菌活性
金黄色葡萄球菌
标准箱
化学
柱色谱法
细菌
高效液相色谱法
色谱法
薄层色谱法
肺炎克雷伯菌
革兰氏阳性菌
琼脂扩散试验
唇形科
传统医学
大肠杆菌
食品科学
生物
生物化学
医学
遗传学
基因
作者
Mohammed Arafat Mohammed,Younis Saadi Saeed,Jassim Fatehi Ali
出处
期刊:PubMed
日期:2023-09-01
卷期号:36 (5): 1435-1442
被引量:1
摘要
Teucrium polium L., a member of the Lamiaceae family. The plant's leaf ingredients were extracted with hexane, ethyl acetate and ethanol, in that order. Column chromatography (CC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC) were used to figure out what phenolic components were in different extracts. Through these methods, three distinct fractions were isolated, originating from ethyl acetate and ethanolic extractions. Further examination of these fractions led to the discovery of 4-Hydroxybenzoic acid, catechol and coumaric acid. The antibacterial activity of isolated components was examined through rigorous testing. To evaluate the efficacy against different types of bacteria, the disc-diffusion assay was employed. This involved testing each fraction against Staphylococcus aureus, a Gram-positive bacterium, as well as four Gram-negative bacteria, including Staphylococcus aureus and Salmonella typhi, Pseudomonas aeruginosa, Klebsiella pneumoniae and Escherichia coli. Fraction (F1) was able to stop the growth of K. pneumoniae, P. aeruginosa, and S. aureus well with an inhibition zone of 19-21 mm. Fraction (F2) showed high activity against all types of bacteria used in this study, with an inhibition zone of 19-23 mm. This study's findings suggest that Teucrium polium holds potential as a valuable source of natural antibacterial phenolic compounds.
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