GPR120 promotes neutrophil control of intestinal bacterial infection

柠檬酸杆菌 生物 柠檬酸杆菌 微生物学 受体 炎症 免疫学 大肠杆菌 肠杆菌科 生物化学 基因
作者
Zheng Zhou,Wenjing Yang,Tianxiang Yu,Yu Yu,Xiaojing Zhao,Yanbo Yu,Chun-Cai Gu,Anthony J. Bilotta,Suxia Yao,Qihong Zhao,George Golovko,Mingsong Li,Yingzi Cong
出处
期刊:Gut microbes [Informa]
卷期号:15 (1) 被引量:1
标识
DOI:10.1080/19490976.2023.2190311
摘要

G-protein coupled receptor 120 (GPR 120) has been implicated in anti-inflammatory functions. However, how GPR120 regulates the neutrophil function remains unknown. This study investigated the role of GPR120 in the regulation of neutrophil function against enteric bacteria. 16S rRNA sequencing was used for measuring the gut microbiota of wild-type (WT) mice and Gpr120-/- mice. Citrobacter rodentium infection and dextran sulfate sodium (DSS)-induced colitis models were performed in WT and Gpr120-/- mice. Mouse peritoneal-derived primary neutrophils were used to determine the neutrophil functions. Gpr120-/- mice showed altered microbiota composition. Gpr120-/- mice exhibited less capacity to clear intestinal Citrobacter rodentium and more severe intestinal inflammation upon infection or DSS insults. Depletion of neutrophils decreased the intestinal clearance of Citrobacter rodentium. GPR120 agonist, CpdA, enhanced WT neutrophil production of reactive oxygen species (ROS) and extracellular traps (NETs), and GPR120-deficient neutrophils demonstrated a lower level of ROS and NETs. CpdA-treated neutrophils showed an enhanced capacity to inhibit the growth of Citrobacter rodentium, which was abrogated by the inhibition of either NETs or ROS. CpdA promoted neutrophil inhibition of the growth of commensal bacteria Escherichia coli O9:H4 and pathobiont Escherichia coli O83:H1 isolated from a Crohn's disease patient. Mechanically, mTOR activation and glycolysis mediated GPR120 induction of ROS and NETs in neutrophils. Additionally, CpdA promoted the neutrophil production of IL-17 and IL-22, and treatment with a conditioned medium of GPR120-activated neutrophils increased intestinal epithelial cell barrier functions. Our study demonstrated the critical role of GPR120 in neutrophils in protection against enteric bacterial invasion.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小胖完成签到,获得积分10
刚刚
唐瑾瑜完成签到,获得积分10
3秒前
哈哈哈哈哈哈完成签到,获得积分10
3秒前
4秒前
5秒前
5秒前
krislang完成签到,获得积分10
6秒前
rundstedt完成签到,获得积分10
7秒前
菠萝谷波发布了新的文献求助10
8秒前
燕子完成签到,获得积分10
9秒前
喆喆发布了新的文献求助20
11秒前
iNk举报王洋求助涉嫌违规
11秒前
12秒前
隐形曼青应助菠萝谷波采纳,获得10
15秒前
16秒前
19秒前
20秒前
rundstedt发布了新的文献求助10
22秒前
22秒前
了一李应助13686682012采纳,获得10
23秒前
InfoNinja应助jack采纳,获得50
23秒前
笑点低的幼旋完成签到,获得积分10
24秒前
甜蜜绿蓉发布了新的文献求助10
27秒前
小刘完成签到,获得积分10
27秒前
111112完成签到,获得积分10
28秒前
充电宝应助喆喆采纳,获得10
29秒前
lwg完成签到,获得积分10
29秒前
29秒前
30秒前
大闲鱼铭一完成签到 ,获得积分10
32秒前
费费发布了新的文献求助10
33秒前
33秒前
34秒前
wbh发布了新的文献求助20
34秒前
34秒前
35秒前
夏日天空完成签到,获得积分20
35秒前
36秒前
喆喆发布了新的文献求助10
38秒前
13686682012完成签到,获得积分10
38秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
The Data Economy: Tools and Applications 1000
Diamonds: Properties, Synthesis and Applications 800
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufen 700
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3096463
求助须知:如何正确求助?哪些是违规求助? 2748430
关于积分的说明 7599646
捐赠科研通 2400115
什么是DOI,文献DOI怎么找? 1273385
科研通“疑难数据库(出版商)”最低求助积分说明 615728
版权声明 598973