Citric acid crosslinked hydroxyethyl tamarind gum-based hydrogel films: A promising biomaterial for drug delivery

This investigation explored citric acid crosslinked hydroxyethyl tamarind gum hydrogel films as a potential biomaterial for drug delivery. Hydroxyethylation of tamarind gum aimed to improve its solubility, swelling, and crosslinking potential. The synthesized hydroxyethylated tamarind gum (HETG) was comprehensively characterized, revealing the presence of hydroxyethyl groups and increased viscosity in comparison to unmodified tamarind gum. The citric acid crosslinked HETG hydrogel films were developed by esterification-crosslinking mechanism. The films were characterized using instrumental techniques and evaluated for total carboxyl content, mechanical properties, swelling behavior, drug loading, drug release, antibacterial activity, hemocompatibility and in vitro wound healing activity. The presence of ester crosslinks and extent of crosslinking was confirmed through total carboxyl content and instrumental analysis. Varying HETG (2-2.5%w/v) and citric acid (1-1.4 %w/v) concentrations resulted in films with tunable mechanical strength, swelling, and drug loading. The films effectively controlled the release of a water-soluble drug (80.87-99.70 % in 24 h) through a non-Fickian diffusion mechanism. The optimized HETG hydrogel film showed antimicrobial activity, hemocompatibility, and support for cell growth, confirming its biocompatibility and potential for wound healing. Citric acid-crosslinked HETG films appear promising for drug delivery to wounds, meriting further in vivo study.