光学
显微镜
黑素体
分辨率(逻辑)
材料科学
生物医学中的光声成像
细胞器
显微镜
光学显微镜
生物医学工程
化学
物理
黑色素
扫描电子显微镜
计算机科学
医学
生物化学
人工智能
作者
Tingting Li,Xinrui Gong,Heng Guo,Lei Xi
出处
期刊:Optics Letters
[Optica Publishing Group]
日期:2024-01-16
卷期号:49 (4): 798-798
摘要
Optical resolution photoacoustic microscopy (OR-PAM) is a hybrid imaging method for visualizing organelles due to the high spatial resolution and abundant optical contrast. Usually, OR-PAM employs high numerical aperture (NA) objectives and high-frequency ultrasonic detectors to resolve three-dimensional (3D) microstructures of cells. Expansion microscopy (ExM) provides a nanoscale resolution by isotropically enlarging cells instead of utilizing ultrahigh NA objectives. In this Letter, we report the development of photoacoustic expansion microscopy (PA-ExM) that combines the advantages of OR-PAM and ExM for 3D organelle imaging using near-infrared light. We evaluate the performance of PA-ExM using label-free melanoma cells, where the image quality of melanosome distributions in expanded cells using a 40× objective is comparable to that of unexpanded cells using an oil-immersed 100× objective. The results suggest that PA-ExM possesses the great potential to study organelles.
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