化学
斑马鱼
线粒体
氰化物
细胞色素c氧化酶
细胞凋亡
自噬
咔唑
荧光
细胞生物学
内生
生物物理学
粘度
程序性细胞死亡
膜电位
生物化学
光化学
生物
物理
基因
无机化学
量子力学
作者
Linlin Han,Wei Pan,Shu-Long He,Mengyu Tang,Xueli Cheng,Xiaoqun Cao,Shi‐Li Shen,Xian-Hong Pang,Yan Zhu
标识
DOI:10.1016/j.bioorg.2023.107023
摘要
Cells of most eukaryotic species contain mitochondria, which play a role in physiological processes such as cellular senescence, metabolism, and autophagy. Viscosity is considered a key marker for many illnesses and is involved in several crucial physiological processes. Cyanide (CN−) can target cytochrome-c oxidase, disrupting the mitochondrial electron transport chain and causing cell death through asphyxiation. In this study, a fluorescent probe named HL-1, which targets mitochondria and measures viscosity and CN− levels, was designed and synthesized. HL-1 is viscosity-sensitive, with a linear correlation coefficient of up to 0.992. In addition, HL-1 was found to change color substantially during a nucleophilic addition reaction with CN−, which has a low detection limit of 47 nM. HL-1 not only detects viscosity and exogenous CN− in SKOV-3 cells and zebrafish but also monitors viscosity changes during mitochondrial autophagy in real time. Furthermore, HL-1 has been used successfully to monitor changes in mitochondrial membrane potential during apoptosis. Endogenous CN− in plant samples was quantified. HL-1 provides new ideas for studying viscosity and CN−.
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