生物
16S核糖体RNA
系统发育树
拉伤
基因组DNA
基因
微生物学
遗传学
解剖
作者
Xiaoquan Wang,Caixia Han,Jing Wang,Siliang Yuan,Dian Jiao,Shanhui Wang,Yaqi Liu,Yan Li,Jingcheng Dai,Shu‐Yang Li,Dongru Qiu
标识
DOI:10.1099/ijsem.0.006249
摘要
A Gram-stain-negative, strictly aerobic and filamentous bacterial strain, designated as DQS-5 T , was isolated from the activated sludge of a municipal sewage treatment plant in Shenzhen, PR China. Optimal growth was observed at 28 °C and pH 7.5. Catalase and oxidase activities were detected. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DQS-5 T was most closely related to the genera Chitinimonas and Chitinivorax (91.0–93.4 % and 92.5 % 16S rRNA gene sequence similarity, respectively) and was close to the member of the family Burkholderiaceae . The complete genome sequence of strain DQS-5 T contains 5 653 844 bp and 57.3 mol% G+C. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between the genome of strain DQS-5 T and those of its close relatives were 75.9–77.2, 19.0–20.3 and 57.2–61.8 %, respectively. Chemotaxonomic analysis of strain DQS-5 T indicated that the sole respiratory quinone was ubiquinone-8, the predominant cellular fatty acids were C 16 : 0 and summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), and the major polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid and aminolipid. The phylogenetic, genotypic, phenotypic and chemotaxonomic data demonstrate that strain DQS-5 T represents a novel species in a novel genus within the family Burkholderiaceae , for which the name Parachitinimonas caeni gen. nov., sp. nov., is proposed. Strain DQS-5 T (=KCTC 92788 T =CCTCC AB 2022320 T ) is the type and only strain of P. caeni .
科研通智能强力驱动
Strongly Powered by AbleSci AI