效应器
生物
线虫
大豆孢囊线虫
线虫感染
甜菜胞囊线虫
分泌物
寄主(生物学)
进化生物学
细胞生物学
生态学
生物化学
作者
B. P. J. Molloy,Damian S. Shin,Jonathan Long,Clément Pellegrin,Beatrice Senatori,Paulo Vieira,Peter Thorpe,Anika Damm,Mariam Ahmad,Katrien Vermeulen,Lida Derevnina,Siyuan Wei,Alexis L. Sperling,Estefany Reyes Estévez,Samuel Bruty,Victor Hugo Moura de Souza,Olaf Prosper Kranse,Tom Maier,Thomas J. Baum,Sebastian Eves‐van den Akker
标识
DOI:10.1101/2024.01.05.574317
摘要
Abstract Plant-parasitic nematodes constrain global food security. During parasitism, they secrete effectors into the host plant from two types of pharyngeal gland cells. These effectors elicit profound changes in host biology to suppress immunity and establish a unique feeding organ from which the nematode draws nutrition. Despite the importance of effectors in nematode parasitism, there has been no comprehensive identification and characterisation of the effector repertoire of any plant-parasitic nematode. To address this, we advance techniques for gland cell isolation and transcriptional analysis to define a stringent annotation of putative effectors for the cyst nematode Heterodera schachtii at three key life-stages. We define 659 effector gene loci: 293 “known” high-confidence homologs of plant-parasitic nematode effectors, and 366 “novel” effectors with high gland cell expression. In doing so we define a comprehensive “effectorome” of a plant-parasitic nematode. Using this effector definition, we provide the first systems-level understanding of the origin, deployment and evolution of a plant-parasitic nematode effectorome. The robust identification of the comprehensive effector repertoire of a plant-parasitic nematode will underpin our understanding of nematode pathology, and hence, inform strategies for crop protection.
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