脚手架
明胶
甲基丙烯酸酯
生物相容性材料
再生(生物学)
恶唑啉
材料科学
多孔性
组织工程
透明质酸
软骨
化学工程
生物医学工程
化学
高分子化学
复合材料
聚合物
解剖
有机化学
聚合
细胞生物学
医学
催化作用
工程类
生物
作者
Sanaz Aliakbarzadeh,Majid Abdouss,Hossein Ali Khonakdar,Abbas Rahdar,Sonia Fathi-karkan
标识
DOI:10.1016/j.molliq.2024.124982
摘要
Gelatin methacrylate (GelMA) is a biocompatible, and biodegradable macromolecule developed from a natural polymer, gelatin, via covalent bonding with methacrylic groups. In this research, GelMA/poly(2-ethyl-2-oxazoline) (PEtOx) hydrogel loaded with Kartogenin (KGN) drug was developed with the aim to regenerate the extracellular matrix of cartilage. The morphology, swelling ratio, degree of hydrophilicity, biodegradability, elastic modulus, blood compatibility, drug release and cytotoxicity were assessed. The hydrogel was found to be hydrophilic, highly porous and biodegradable, with 92 % porosity and ∼ 43 % weight loss within 14 days. Mechanical testing revealed that the elastic modulus of GelMA (20 wt%)/PEtOx (45 wt%) was close to that of cartilage. Good blood compatibility and sustained drug release in the damaged cartilage tissue environment were also detected. According to flow cytometry and MTT tests, due to the presence of KGN, up to 75 % of the cells can survive and easily adhere to the developed scaffold. The results confirm the ability of GelMA/PEtOx hydrogel containing KGN to increase cell adhesion by preserving cell population combined with minimal cytotoxicity, which may be advantageous for use in cartilage regeneration following in-vivo implantation.
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