间充质干细胞
脂肪组织
纤维蛋白
脚手架
再生(生物学)
移植
干细胞
病理
组织工程
生物医学工程
化学
医学
细胞生物学
生物
外科
免疫学
生物化学
作者
Thao Duy Huynh,H K Nguyen,A M Inchingolo,Ha Le Bao Tran,G Dipalma,A Mancini,K Cao Diem Nguyen,M G Balzanelli,P Distratis,R Lazzaro,T A Nguyen Ho,E M Serlenga,V Hung Pham,G Romanos,B T Nguyen,B Rapone,F Inchingolo,C Isacco Gargiulo,T Cong Tran
出处
期刊:PubMed
日期:2023-04-01
卷期号:27 (8): 3670-3680
被引量:3
标识
DOI:10.26355/eurrev_202304_32161
摘要
Our study aimed to evaluate the effect of soft tissue regeneration in nude mice using grafts made from the combination of adipocytes from fat tissue mesenchymal stem cells and fibrin gel from peripheral blood.Mesenchymal stem cells were isolated from adipose tissue and identified according to ISCT criteria. The scaffold used was fibrin obtained from peripheral blood. The grafts in this study were generated by transferring mesenchymal stem cells onto a fibrin scaffold. Two types of grafts, the research sample (fibrin scaffold containing adipocytes differentiated from mesenchymal stem cells) and the control sample (fibrin scaffold only), were grafted under the dorsal skin of the same mouse. After each research period, samples were collected and evaluated by histological methods to observe the existence and growth of cells inside the grafts.The results showed that the study group's graft integrated better within the tissue when compared with the control group. In addition, the grafts in the study group showed the presence of cells with characteristic morphology of adipocytes one week after transplantation. In contrast, control samples showed dimorphous shapes and features mainly composed of non-homogenous fragments.These initial conclusions might be considered a first step in generating safe bio-compatible engineered grafts specifically usable in post-traumatic tissue regeneration procedures.
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