Recovery of the phenolic compounds from artificial cultivated Sanghuangporus vaninii using a green method and biological properties of phenolic extract in vitro

Summary Modern research has demonstrated that Sanghuangporus vaninii ( S . vaninii ) has significant therapeutic value, and phenolic compounds are one of its most active ingredients. In this study, deep eutectic solvents (DES) were used to extract the phenolic components from S . vaninii using response surface methodology. The optimal conditions were obtained with a water content of 20%, a material‐to‐liquid ratio of 1:60 g mL −1 , an extraction temperature of 70°C and an extraction time of 34 min. Under these conditions, the total phenolic content reached 9.71 ± 0.69 mg g −1 , which was higher than the conventional method (4.23 ± 0.44 mg g −1 ). The phenolic extract was then purified by HPD‐100, and the purified phenolic extracts exhibited distinct antioxidant, antibacterial and hypoglycaemic activities. Twenty‐five phenolic compounds were identified using UPLC‐Triple‐TOF‐MS/MS. These data implied that the integrated action of the identified phenolic compounds had a decisive influence on the biological activity of the extracts, and this study furnished the basis for the utilisation of S . vaninii .