Abstract 1538 Phosphatidylserine regulates plasma membrane repair through the assembly of tetraspanin-enriched macrodomains

The integrity of the plasma membrane is critical to cell function and survival. Cells have developed multiple mechanisms to repair damaged plasma membranes. A key process during plasma membrane repair is to limit the size of the damage, which is facilitated by the presence of Tetraspanin-enriched rings surrounding damage sites. Here, we identify phosphatidylserine-enriched rings surrounding damaged sites of the plasma membrane, resembling the Tetraspanin-enriched rings. Importantly, the formation of both the phosphatidylserine- and Tetraspanin-enriched rings requires phosphatidylserine and its transfer proteins ORP5 and ORP9.