驯化
增强子
染色质
调节器
生物
等位基因
基因表达调控
计算生物学
细胞生物学
遗传学
基因
基因表达
作者
Feng Bai,Peng Shu,Heng Deng,Yi Wu,Yao Chen,Mengbo Wu,Tao Ma,Yang Zhang,Julien Pirrello,Zhengguo Li,Yiguo Hong,Mondher Bouzayen,Mingchun Liu
标识
DOI:10.1038/s41467-024-47292-7
摘要
Abstract Steroidal glycoalkaloids (SGAs) are major plant defense metabolites against pests, while they are considered poisonous in food. The genetic basis that guides negative selection of SGAs production during tomato domestication remains poorly understood. Here, we identify a distal enhancer, GAME Enhancer 1 ( GE1 ), as the key regulator of SGAs metabolism in tomato. GE1 recruits MYC2-GAME9 transcriptional complex to regulate the expression of GAME cluster genes via the formation of chromatin loops located in the neighboring DNA region. A naturally occurring GE1 76 allelic variant is found to be more active in stimulating GAME expression. We show that the weaker GE1 allele has been the main driver for selecting reduced SGAs levels during tomato domestication. Unravelling the “TFs-Enhancer-Promoter” regulatory mechanism operating in SGAs metabolism opens unprecedented prospects for SGAs manipulation in Solanaceae via precision breeding strategies.
科研通智能强力驱动
Strongly Powered by AbleSci AI