间充质干细胞
胰岛素
生物
祖细胞
干细胞
移植
干细胞移植修复关节软骨
细胞分化
内分泌学
细胞疗法
内科学
癌症研究
免疫学
细胞生物学
成体干细胞
医学
生物化学
基因
作者
So-Yeon Kim,Ye-Ryung Kim,Woo‐Jae Park,Han Su Kim,Sung‐Chul Jung,So‐Youn Woo,Inho Jo,Kyung‐Ha Ryu,Joo‐Won Park
标识
DOI:10.1016/j.diff.2015.08.001
摘要
Tonsil-derived (T-) mesenchymal stem cells (MSCs) display mutilineage differentiation potential and self-renewal capacity and have potential as a banking source. Diabetes mellitus is a prevalent disease in modern society, and the transplantation of pancreatic progenitor cells or various stem cell-derived insulin-secreting cells has been suggested as a novel therapy for diabetes. The potential of T-MSCs to trans-differentiate into pancreatic progenitor cells or insulin-secreting cells has not yet been investigated. We examined the potential of human T-MSCs to trans-differentiate into pancreatic islet cells using two different methods based on β-mercaptoethanol and insulin–transferin–selenium, respectively. First, we compared the efficacy of the two methods for inducing differentiation into insulin-producing cells. We demonstrated that the insulin–transferin–selenium method is more efficient for inducing differentiation into insulin-secreting cells regardless of the source of the MSCs. Second, we compared the differentiation potential of two different MSC types: T-MSCs and adipose-derived MSCs (A-MSCs). T-MSCs had a differentiation capacity similar to that of A-MSCs and were capable of secreting insulin in response to glucose concentration. Islet-like clusters differentiated from T-MSCs had lower synaptotagmin-3, -5, -7, and -8 levels, and consequently lower secreted insulin levels than cells differentiated from A-MSCs. These results imply that T-MSCs can differentiate into functional pancreatic islet-like cells and could provide a novel, alternative cell therapy for diabetes mellitus.
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