Preparation, characterization and antibacterial properties of 6-deoxy-6-arginine modified chitosan

In this study, 6-deoxy-6-arginine modified chitosan (DAC), was synthesized and characterized by Fourier Transform Infrared Spectroscopy (FTIR), 1H and 13C nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and elemental analysis. The arginine was grafted onto C6 groups of chitosan. Antibacterial activity of DAC against gram-negative bacteria Escherichia coli (E. coli) and gram-positive bacteria Staphylococcus aureus (S. aureus) were investigated at concentration between 0.02 mg/mL and 10 mg/mL. Cell viability assessment was estimated in vitro with Caco-2 and L929 cells. Water solubility of DAC at different pH was also evaluated. The results showed that the minimum inhibitory concentration (MICs) of DAC against S. aureus and E. coli were 0.078 mg/mL and 0.312 mg/mL, respectively. The minimum bactericidal concentration (MBC) against S. aureus and E. coli was 0.625 mg/mL. The cytotoxicity of chitosan and DAC was not significantly different. It demonstrated that DAC might be a potential safe antibacterial agent.