[Discussion on calculation method of detection limit and quantitative limit of occupational health biological monitoring method].

Objective: To discuss calculation method of detection limit and quantitative limit of occupational health biological monitoring. Methods: The detection limit and the quantitative limit of phenyl glyoxylic acid and Mandelic acid were calculated by using three different methods of IUPAC, NIOSH and OSHA respectively. Results: The IUPAC, NIOSH and OSHA methods were used to calculate the detection limit and the quantitative limit of the phenyl glyoxylic acid and Mandelic acid, and the results are different. Conclusion: To calculate the detection limit and quantitative limit of occupational health biological monitoring methods, the standard curve method is adopted to ensure that the rate of detection in the vicinity of detection limit and more than 75% of the quantitative limits are used.目的： 探讨职业卫生生物监测方法检出限和定量限的计算方法。 方法： 通过IUPAC、NIOSH、OSHA三种不同方法分别计算苯乙醛酸和苯乙醇酸的检出限和定量限。 结果： 采用IUPAC、NIOSH、OSHA三种不同方法计算的苯乙醛酸和苯乙醇酸的检出限和定量限各不相同。 结论： 计算职业卫生生物监测方法检出限和定量限时，为保证检出限附近的检出率及定量限附近有75%以上的回收率应采用标准曲线法，通过一系列浓度加标的方式来计算检出限和定量限。.