MAPK/ERK通路
肿瘤坏死因子α
免疫系统
一氧化氮
一氧化氮合酶
NF-κB
化学
αBκ
双孢蘑菇
白细胞介素
磷酸化
信号转导
生物
细胞生物学
生物化学
细胞因子
免疫学
食品科学
有机化学
蘑菇
作者
Xiaotong Zhao,Peilin Hou,Huijie Xin,Yanqing Zhang,Aimin Zhou,Chang‐Jiang‐Sheng Lai,Junbo Xie
标识
DOI:10.1016/j.ijbiomac.2019.10.148
摘要
In the present study, a glucogalactomanan polysaccharide isolated from Agaricus bisporus named as TJ3 was investigated its immunomodulatory effects and molecular mechanisms in RAW 264.7 cells. Functional analysis showed TJ3 could inhibit the proliferation of RAW 264.7 cells in a certain concentration range. Moreover, TJ3 treatment increased the expression of inducible nitric oxide synthase (iNOS), which was responsible for increasing nitric oxide (NO) production. TJ3 also increased the expression of cyclooxygenase-2 (COX-2) and the mRNA levels of interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α) and COX-2 in RAW 264.7 cells. In addition, further elucidation of molecular mechanisms showed that ERK/MAPK and IκB/NFκB pathways were activated by TJ3 treatment via increased phosphorylation levels of ERK1/2 and IκB-α, respectively. These findings indicated that TJ3 had a huge promising immunostimulatory activity and its induced immune responses probably through stimulating the ERK/MAPK and IκB/NFκB pathways. Our results demonstrate that TJ3 may be used as a potent immune modulator.
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