医学
尿
生物标志物
尿路上皮癌
小瓶
泌尿科
内科学
癌症
色谱法
膀胱癌
化学
生物化学
作者
Ying‐Pei Hsu,Hung‐Wei Yang,Nan‐Si Li,Ying‐Tzu Chen,Hao‐Han Pang,See‐Tong Pang
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2020-03-12
卷期号:5 (4): 928-935
被引量:9
标识
DOI:10.1021/acssensors.9b02013
摘要
The incidence and 5 year recurrence rate of urothelial carcinomas (UCs), including UC of the bladder (UCB) and upper urinary tract UC (UTUC), have increased annually. There is a great need for a simple and fast point-of-care (POC) test for early diagnosis and amelioration in the survival rate. We present a POC test comprising a new vial-immunosensor, nanoenzyme, and iPhone 7 plus, which detects and quantifies the new biomarker FXYD domain-containing ion transport regulator 3 (FXYD3) in human urine for specific UC screening, tumor-grade classification, and postoperative monitoring by the grayscale value of the photograph taken. The performance of the proposed POC test was then verified using urine from 4 healthy people, 40 UCB patients (10 patients were low-grade and 30 patients were high-grade), and 13 UTUC patients (2 patients were low-grade and 11 patients were high-grade), confirming the accuracy and specificity by comparing the results with those obtained by enzyme-linked immunosorbent assay (ELISA). Moreover, we also designed a correction method that can make the grayscale values calculated by different smartphones close to the values calculated by iPhone 7 plus, resulting in the POC test enabling simple, fast, universal, and portable testing, data storage, and sharing for personal UCs screening and postoperative monitoring.
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