衰老
生物
ERCC1公司
DNA损伤
DNA修复
早熟
细胞生物学
分子生物学
癌症研究
遗传学
核苷酸切除修复
DNA
基因
作者
Matthew J. Yousefzadeh,Jing Zhao,Christina Bukata,Erin A. Wade,Sara J. McGowan,Luise Angelini,Michael P. Bank,Aditi U. Gurkar,Collin A. McGuckian,Mariah F. Calubag,Jonathan Kato,Christin E. Burd,Paul D. Robbins,Laura J. Niedernhofer
出处
期刊:Aging Cell
[Wiley]
日期:2020-01-25
卷期号:19 (3)
被引量:193
摘要
Abstract Senescent cells accumulate with age in vertebrates and promote aging largely through their senescence‐associated secretory phenotype (SASP). Many types of stress induce senescence, including genotoxic stress. ERCC1‐XPF is a DNA repair endonuclease required for multiple DNA repair mechanisms that protect the nuclear genome. Humans or mice with reduced expression of this enzyme age rapidly due to increased levels of spontaneous, genotoxic stress. Here, we asked whether this corresponds to an increased level of senescent cells. p16 Ink4a and p21 Cip1 mRNA were increased ~15‐fold in peripheral lymphocytes from 4‐ to 5‐month‐old Ercc1 −/∆ and 2.5‐year‐old wild‐type (WT) mice, suggesting that these animals exhibit a similar biological age. p16 Ink4a and p21 Cip1 mRNA were elevated in 10 of 13 tissues analyzed from 4‐ to 5‐month‐old Ercc1 −/∆ mice, indicating where endogenous DNA damage drives senescence in vivo. Aged WT mice had similar increases of p16 Ink4a and p21 Cip1 mRNA in the same 10 tissues as the mutant mice. Senescence‐associated β–galactosidase activity and p21 Cip1 protein also were increased in tissues of the progeroid and aged mice, while Lamin B1 mRNA and protein levels were diminished. In Ercc1 −/Δ mice with a p16 Ink4a luciferase reporter, bioluminescence rose steadily with age, particularly in lung, thymus, and pancreas. These data illustrate where senescence occurs with natural and accelerated aging in mice and the relative extent of senescence among tissues. Interestingly, senescence was greater in male mice until the end of life. The similarities between Ercc1 −/∆ and aged WT mice support the conclusion that the DNA repair‐deficient mice accurately model the age‐related accumulation of senescent cells, albeit six‐times faster.
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