胚乳
生物
合子
胚胎
拟南芥
胚珠
细胞生物学
遗传学
异位表达
突变体
拟南芥
细胞命运测定
西力克
胚胎发生
植物
基因
转录因子
作者
Baojian Chen,Lena Maas,Duarte D. Figueiredo,Yu Zhong,Ricardo Souza Reis,Mengran Li,Anneke Horstman,Tjitske Riksen,Mieke Weemen,Hang Liu,Charlotte Siemons,Shaojiang Chen,Gerco C. Angenent,Kim Boutilier
标识
DOI:10.1073/pnas.2201761119
摘要
The BABY BOOM (BBM) AINTEGUMENTA-LIKE (AIL) AP2/ERF domain transcription factor is a major regulator of plant cell totipotency, as it induces asexual embryo formation when ectopically expressed. Surprisingly, only limited information is available on the role of BBM during zygotic embryogenesis. Here we reexamined BBM expression and function in the model plant Arabidopsis thaliana ( Arabidopsis ) using reporter analysis and newly developed CRISPR mutants. BBM was expressed in the embryo from the zygote stage and also in the maternal (nucellus) and filial (endosperm) seed tissues. Analysis of CRISPR mutant alleles for BBM ( bbm-cr ) and the redundantly acting AIL gene PLETHORA2 ( PLT2 ) ( plt2-cr ) uncovered individual roles for these genes in the timing of embryo progression. We also identified redundant roles for BBM and PLT2 in endosperm proliferation and cellularization and the maintenance of zygotic embryo development. Finally, we show that ectopic BBM expression in the egg cell of Arabidopsis and the dicot crops Brassica napus and Solanum lycopersicon is sufficient to bypass the fertilization requirement for embryo development. Together these results highlight roles for BBM and PLT2 in seed development and demonstrate the utility of BBM genes for engineering asexual embryo development in dicot species.
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