Study on the effects of alternating capacitive electric fields with different frequencies on promoting wound healing

Some researches to facilitate wound healing by using electrical stimulation are based on electric current stimulation, which may cause secondary damage and the imbalance of the microenvironment in vivo. In this study, alternating capacitive electric field (ACEF) was applied via a self-designed system so as to avoid direct contact with cells and to maintain stable microenvironment for cell growth. The influences of 58 ​mV/mm ACEFs with various frequencies of 10, 60 and 110 ​Hz on epidermal cells, fibroblasts and macrophages which involve in wound healing were comprehensively explored. The results suggested that ACEFs of 10, 60 and 110 ​Hz all significantly promoted the proliferation of human dermal fibroblasts (HDFs) and human epidermal keratinocyte cell line (HaCaT) cells, and 60 ​Hz ACEF furtherly accelerated the migration of these two kinds of cells. Moreover, ACEFs of all different studied frequencies facilitated M2-type polarization of macrophages, and YAP/TAZ expression of macrophages were enhanced under the stimulations of 10 and 60 ​Hz ACEFs. The enhancements in cell activity, migration rate and M2-type polarizability indicated that 58 ​mV/mm ACEFs especially at 60 ​Hz possessing potentially affirmative applications for wound healing without the risks of secondary damage and microenvironment imbalance.