同色
乙酰胆碱受体
烟碱乙酰胆碱受体
烟碱激动剂
生物
爪蟾
乙酰胆碱
配体门控离子通道
离子通道
血清素
受体
异源表达
兴奋剂
半胱氨酸环受体
新烟碱
药理学
作者
Eleanor L. Mitchell,Franco Viscarra,Isabel Bermúdez,Joseph A. Hawkins,Jim Goodchild,Andrew K. Jones
标识
DOI:10.1016/j.pestbp.2022.105055
摘要
Insect nicotinic acetylcholine receptors (nAChRs) are molecular targets of highly effective insecticides such as neonicotinoids. Functional expression of these receptors provides useful insights into their functional and pharmacological properties. Here, we report that the α5 nAChR subunit of the honey bee, Apis mellifera, functionally expresses in Xenopus laevis oocytes, which is the first time a homomeric insect nAChR has been robustly expressed in a heterologous system without the need for chaperone proteins. Using two-electrode voltage-clamp electrophysiology we show that the α5 receptor has low sensitivity to acetylcholine with an EC50 of 2.37 mM. However, serotonin acts as an agonist with a considerably lower EC50 at 119 μM that is also more efficacious than acetylcholine in activating the receptor. Molecular modelling indicates that residues in the complementary binding site may be involved in the selectivity towards serotonin. This is the first report of a ligand-gated ion channel activated by serotonin from an insect and phylogenetic analysis shows that the α5 subunit of A. mellifera and other non-Dipteran insects, including pest species, belong to a distinct subgroup of subunits, which may represent targets for the development of novel classes of insecticides.
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