空间光调制器
光学
投影(关系代数)
显微镜
材料科学
栅栏
空间频率
数字微镜装置
计算机科学
薄层荧光显微镜
瓶颈
光场
图像分辨率
物理
算法
扫描共焦电子显微镜
嵌入式系统
作者
Kai Wen,Xiang Fang,Ying Ma,Min Liu,Sha An,Juanjuan Zheng,Tomasz Kozacki,Peng Gao
出处
期刊:Optics Letters
[The Optical Society]
日期:2022-04-29
卷期号:47 (11): 2666-2666
被引量:19
摘要
Structured illumination microscopy (SIM) has been widely used in biological research due to its merits of fast imaging speed, minimal invasiveness, super-resolution, and optical sectioning imaging capability. However, the conventional SIM that uses a spatial light modulator (SLM) for fringe projection often has a limited imaging field of view. Herein, we report a large-field SIM technique that combines a 2D grating for fringe pattern projection and an SLM for selecting fringe orientation and performing phase shifting digitally. The proposed SIM technique breaks the bottleneck of fringe number limited by the digital projection devices, while maintaining the advantage of high-speed (digital) phase shifting of conventional SIM. The method avoids the pixilation and dispersion effects of the SLMs. Finally, a 1.8-fold resolution enhancement in a large field of 690 × 517 µm2 under a 20×/NA0.75 objective is experimentally demonstrated. The proposed technique can be widely applied to biology, chemistry, and industry.
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