斑马鱼
生物
侧线
增强剂陷阱
绿色荧光蛋白
细胞生物学
增强子
器官发生
转基因
发育生物学
耳泡
细胞培养
基因表达
原位杂交
基因
遗传学
作者
Chunxin Fan,Yajing Ouyang,Xiaoyi Yuan,Jian Wang
标识
DOI:10.1016/j.gep.2022.119231
摘要
Zebrafish lateral line system which is derived from neurogenic placodes has become a popular model for developmental biology since its formation involves cell migration, pattern formation, organogenesis, and hair cell regeneration. Transgenic lines play a crucial role in lateral line system study. Here, we identified an enhancer trap transgenic zebrafish line Et(gata2a:EGFP)189b (ET189b for short), which expressed enhanced green fluorescent protein (EGFP) in the pituitary, otic, and lateral line placodes and their derivatives. Especially, in neuromast, the accessory cells rather than hair cells were labeled by EGFP. Furthermore, we found the Tol2 transposon construct is integrated at the proximal upstream region of six2b gene locus. And EGFP expression of ET189b closely reflects the expression of endogenous six2b during development and after dkk1b over-expression. Taken together, our results indicated that ET189b is an ideal line for research on lateral line development and regulation of six2b expression.
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