Polydopamine-based nanozyme with dual-recognition strategy-driven fluorescence-colorimetric dual-mode platform for Listeria monocytogenes detection

Development of a simple and efficient dual-mode analytical technique with the built-in cross reference correction feature is benefit to achieve the highly accurate detection of the target pollutants and avoid the false-positive outputs in environmental media. Here, we synthesized a Fe-doped polydopamine ([email protected])-based nanozyme with prominent peroxide-mimetic enzyme activity and high fluorescence emission ability. On this basis, we designed a dual-recognition strategy-driven fluorescence-colorimetric dual-mode detection platform, consisting of Listeria monocytogenes (L. monocytogenes) recognition aptamer-modified [email protected] (apt/[email protected]) and vancomycin-functionalized Fe3O4 (van/Fe3O4), for L. monocytogenes. Owing to van/Fe3O4-powered magnetic separation, there was a L. monocytogenes concentration-dependent fluorescence enhancement of apt/[email protected] for performing fluorescence assay in the precipitate. In this case, the prominent peroxide-mimetic enzyme activity of the residual apt/[email protected] in the precipitation could catalyze H2O2 to further oxidate colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB, which displayed a L. monocytogenes concentration-dependent absorbance enhancement for carrying out colorimetric assay as well. As a result, a fluorescence-colorimetric dual-mode analytical platform was proposed to successfully detect the residual L. monocytogenes in real environmental media with acceptable results. This work showed the great prospects by integrating dual-recognition strategy into fluorescence nanozyme to develop efficient and reliable dual-mode analytical platforms for safeguarding environmental health.