间质细胞
生物
永生化细胞系
细胞生物学
肿瘤转化
干细胞
同源盒蛋白纳米
胚胎干细胞
羊膜上皮细胞
免疫学
癌症研究
病理
成体干细胞
细胞培养
诱导多能干细胞
癌变
医学
遗传学
癌症
基因
作者
Shiwu Zhang,Imelda Mercado‐Uribe,Anil K. Sood,Robert C. Bast,Jinsong Liu
出处
期刊:Genes & Cancer
[Impact Journals, LLC]
日期:2016-04-08
卷期号:7 (3-4): 60-72
被引量:36
标识
DOI:10.18632/genesandcancer.102
摘要
Stromal cells are generally considered to be derived primarily from the host's normal mesenchymal stromal cells or bone marrow. However, the origins of stromal cells have been quite controversial. To determine the role of polyploidy in tumor development, we examined the fate of normal mullerian epithelial cells during the immortalization and transformation process by tracing the expression of SV40 large T antigen. Here we show that immortalized or HRAS-transformed mullerian epithelial cells contain a subpopulation of polyploid giant cells that grow as multicellular spheroids expressing hematopoietic markers in response to treatment with CoCl2. The immortalized or transformed epithelial cells can transdifferentiate into stromal cells when transplanted into nude mice. Immunofluorescent staining revealed expression of stem cell factors OCT4, Nanog, and SOX-2 in spheroid, whereas expression of embryonic stem cell marker SSEA1 was increased in HRAS-transformed cells compared with their immortalized isogenic counterparts. These results suggest that normal mullerian epithelial cells are intrinsically highly plastic, via the formation of polyploid giant cells and activation of embryonic stem-like program, which work together to promote the coevolution of neoplastic epithelial cells and multiple lineage stromal cells.
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