平移(音频)
噬菌体展示
计算生物学
抗体
克隆(编程)
双特异性抗体
计算机科学
生物
遗传学
单克隆抗体
古生物学
缩放
程序设计语言
镜头(地质)
作者
Zhongyu Zhu,Dimiter S. Dimitrov
标识
DOI:10.1007/978-1-59745-554-1_6
摘要
Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In support to the antibody-based therapeutics, the advent of recent technologies has made human antibody screening and production progressively more economic. Among those technologies, phage-display antibody library has been successfully applied in the antibody-based drug development both as fully human antibody sources and tools for antibody engineering. Building up a high-quality antibody library with a large library size and high diversity has been crucial for successful isolation of antibodies. Here we describe an efficient strategy for the construction of a large naïve phage-display human Fab library with one-step cloning. Optimization of each key step is extensively discussed and simplified protocols for library panning and Fab production are also described.
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