生物
干细胞
毛囊
内斯汀
细胞生物学
干细胞标记物
诱导多能干细胞
细胞分化
成体干细胞
神经干细胞
胚胎干细胞
生物化学
基因
作者
Kyoumi Shirai,Koya Obara,Natsuko Tohgi,Aiko Yamazaki,Ryoichi Aki,Yuko Hamada,Nobuko Arakawa,Shree Ram Singh,Robert M. Hoffman,Yasuyuki Amoh
出处
期刊:Tissue & Cell
[Elsevier]
日期:2019-08-01
卷期号:59: 33-38
被引量:11
标识
DOI:10.1016/j.tice.2019.06.001
摘要
Hair-follicle-associated pluripotent (HAP) stem cells reside in the upper part of the bulge area of the the hair follicle. HAP stem cells are nestin-positive and keratin 15-negative and have the capacity to differentiate into various types of cells in vitro. HAP stem cells are also involved in nerve and spinal cord regeneration in mouse models. Recently, it was shown that the DNA-damage response in non-HAP hair follicle stem cells induces proteolysis of type-XVII collagen (COL17A1/BP180), which is involved in hair-follicle stem-cell maintenance. COL17A1 proteolysis stimulated hair-follicle stem-cell aging, characterized by the loss of stemness signatures and hair-follicle miniaturization associated with androgenic alopecia. In the present study, we demonstrate that HAP stem cells co-express nestin and COL17A1 in vitro and in vivo. The expression of HAP stem cell markers (nestin and SSEA1) increased after HAP stem-cell colonies were formed, then decreased after differentiation to epidermal keratinocytes. In contrast COL17A1 increased after differentiation to epidermal keratinocytes. These results suggest that COL17A1 is important in differentiation of HAP stem cells.
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