胶束
纳米载体
结合
化学
生物物理学
荧光寿命成像显微镜
荧光
水动力半径
药物输送
吲哚青绿
肿瘤微环境
组合化学
有机化学
水溶液
肿瘤细胞
外科
数学分析
物理
癌症研究
生物
医学
量子力学
数学
作者
Pengyong Li,Mengmeng Sun,Zhongjie Xu,Xinyu Liu,Wenguo Zhao,Weiping Gao
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2018-10-23
卷期号:19 (11): 4472-4479
被引量:49
标识
DOI:10.1021/acs.biomac.8b01368
摘要
Self-assembly of site-selective protein-polymer conjugates into stimuli-responsive micelles is interesting owing to their potential biomedical applications, ranging from molecular imaging to drug delivery, but remains a significant challenge. Herein we report a method of site-selective in situ growth-induced self-assembly (SIGS) to synthesize site-specific human serum albumin-poly(2-(diisopropylamino)ethyl methacrylate) (HSA-PDPA) conjugates that can in situ self-assemble into pH-responsive micelles with tunable morphologies. Indocyanine green (ICG) was selectively loaded into the core of sphere-like HSA-PDPA micelles to form pH-responsive fluorescence nanoprobes. The nanoprobes rapidly dissociated into protonated individual unimers at a transition pH of around 6.5, that is the extracellular pH of tumors, which resulted in a sharp fluorescence increase and markedly enhanced cellular uptake. In a tumor-bearing mouse model, they exhibited greatly enhanced tumor fluorescence imaging as compared to ICG alone and pH-nonresponsive nanoprobes. These findings suggest that pH-responsive and site-selective protein-polymer conjugate micelles synthesized by SIGS are promising as a new class of tumor microenvironment-responsive nanocarriers for enhanced tumor imaging and therapy.
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