二氯荧光素
活力测定
链脲佐菌素
细胞凋亡
化学
氧化应激
膜联蛋白
p38丝裂原活化蛋白激酶
MTT法
免疫印迹
MAPK/ERK通路
分子生物学
细胞生物学
内分泌学
信号转导
生物
生物化学
糖尿病
基因
作者
Haijun Zhang,Chunping Zhu,Zhe Sun,Yan XiaoGuang,Huihui Wang,Haibo Xu,Jiani Ma,Yanrong Zhang
出处
期刊:Life Sciences
[Elsevier]
日期:2019-09-01
卷期号:233: 116732-116732
被引量:13
标识
DOI:10.1016/j.lfs.2019.116732
摘要
Linderane, an important bioactive compound in Linderae, improved glucose and lipid metabolism in ob/ob mice. However, the effect of linderane on streptozotocin (STZ)-induced oxidative damage in INS-1 cells remains unclear. INS-1 cells were pre-treated with different doses of linderane for 2 h and then treated with 3 mM STZ for 12 h. Cell viability was determined by MTT assay. Cell apoptosis was detected using an Annexin V-FITC Apoptosis Detection Kit. The level of intracellular ROS was determined using dichlorofluorescein-diacetate (DCFH-DA). The activities of insulin secretion, SOD, catalase (CAT) and GPx were measured using ELISA kits. The expression levels of bax, bcl-2, p38, p-p38, nuclear Nrf2 and HO-1 were measured using western blot. The results showed that STZ-caused inhibitory effects on cell viability and insulin secretion were mitigated by linderane. Furthermore, linderane inhibited apoptosis and oxidative stress in STZ-induced INS-1 cells. Finally, linderane suppressed the activation of p38 MAPK pathway, as well as enhanced the activation of Nrf2 pathway in STZ-induced INS-1 cells. Activation of p38 MAPK pathway or inhibition of Nrf2 significantly reversed the protective effects of linderane against STZ-induced ROS production and cell apoptosis. The protective effects of linderane on STZ-induced INS-1 cells might be attributed to the inhibition of p38 MAPK and activation of Nrf2 pathway.
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