Enzymatic oil-degumming by a novel microbial phospholipase

European Journal of Lipid Science and TechnologyVolume 103, Issue 6 p. 333-340 Research PaperFull Access Enzymatic oil-degumming by a novel microbial phospholipase Kim Clausen, Corresponding Author Kim Clausen [email protected] Future Industries, Research & Development, Novozymes A/S, Smoermosevej 9, 6BS.99, DK-2880 Bagsvaerd, Denmark. Phone: +45-4442-2835, Fax: +45-4444-4096Future Industries, Research & Development, Novozymes A/S, Smoermosevej 9, 6BS.99, DK-2880 Bagsvaerd, Denmark. Phone: +45-4442-2835, Fax: +45-4444-4096Search for more papers by this author Kim Clausen, Corresponding Author Kim Clausen [email protected] Future Industries, Research & Development, Novozymes A/S, Smoermosevej 9, 6BS.99, DK-2880 Bagsvaerd, Denmark. Phone: +45-4442-2835, Fax: +45-4444-4096Future Industries, Research & Development, Novozymes A/S, Smoermosevej 9, 6BS.99, DK-2880 Bagsvaerd, Denmark. Phone: +45-4442-2835, Fax: +45-4444-4096Search for more papers by this author First published: 18 June 2001 https://doi.org/10.1002/1438-9312(200106)103:6<333::AID-EJLT333>3.0.CO;2-FCitations: 104AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Phospholipase A-mediated oil-degumming is a well-established process step (Enzy-Max®) in physical refining of vegetable oils (rape seed, soy bean, sunflower seed). A screening programme for microbial phospholipases of type A has been carried out. The target has been to develop a stable and robust phospholipase with optimal oil-degumming performance in the pH-range 4—5 and in the temperature range 30— 70 °C. One phospholipase of type A1 from Fusarium oxysporum, given the trade name Lecitase® Novo, has been studied in detail. Some of the characteristics of this novel microbial phospholipase in the oil-degumming application are: pH optimum ∼5, temperature optimum 40—45 °C. In laboratory tests the new phospholipase Lecitase® Novo has proven to be superior to porcine pancreatic Lecitase® 10L and other phospholipases with respect to oil-degumming performance, and it has proven to be suited for degumming of different oil qualities ranging from water-degummed to crude oil. A further advantage is that the new phospholipase acts at very low water content, which will make the problematic sludge recycling in the EnzyMax® process superfluous. 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