生物
枯草芽孢杆菌
支原体
柠檬酸
微生物学
基因
牛分枝杆菌
分枝杆菌
芽孢杆菌(形态)
基因表达
遗传学
结核分枝杆菌
细菌
肺结核
医学
病理
作者
Vladimir Pelicic,Jean‐Marc Reyrat,Brigitte Gicquel
标识
DOI:10.1128/jb.178.4.1197-1199.1996
摘要
Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycobacteria.
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