桑格测序
移液管
协议(科学)
计算机科学
DNA测序
模板
化学
DNA
计算生物学
生物
生物化学
医学
病理
物理化学
程序设计语言
替代医学
作者
Jürgen Zimmermann,H. Voß,Christian Schwager,J. Stegemann,Wilhelm Ansorge
出处
期刊:FEBS Letters
[Wiley]
日期:1988-06-20
卷期号:233 (2): 432-436
被引量:59
标识
DOI:10.1016/0014-5793(88)80477-0
摘要
The protocol for Sanger dideoxy chain termination reactions in DNA sequencing is tedious and prone to errors due to the repetitive character of the pipetting steps. An industrial robot, with the addition of a few simple parts, was programmed to automate the dideoxy sequencing reactions. The system is set up in a short time for routine operation and it is faster and more reliable than a human operator. It is flexible and allows variations and optimization of the standard procedure. Disposable microtiter plates at a controlled temperature are used. In one reaction cycle (about 50 min) up to 48 templates are processed. Up to 450 bases were resolved in automated DNA sequencing on samples prepared by the robot. The protocol is applicable to fluorescent as well as to radioactive labeling.
科研通智能强力驱动
Strongly Powered by AbleSci AI