生物
拟南芥
基因
胚芽鞘
北方斑点
南方斑点
转基因
转基因作物
拟南芥
油菜素甾醇
遗传学
基因表达
植物
突变体
作者
Yazhong Tao,Jun Zheng,Zuomi Xu,Xiaohong Zhang,Ke Zhang,Guoying Wang
出处
期刊:Plant Science
[Elsevier]
日期:2004-06-09
卷期号:167 (4): 743-751
被引量:51
标识
DOI:10.1016/j.plantsci.2004.05.012
摘要
Brassinosteroids (BRs) are natural plant growth regulators required for plant growth and development. Physiological studies have demonstrated that exogenous BRs induced the elongation of coleoptiles and mesocotyls in maize. However, little is known about the function of endogenous BRs in maize or other grass species. The Arabidopsis DWF1/DIM is a bifunctional protein which catalyses both the Δ24 isomeration, and subsequently, the Δ24(25) reduction in BRs biosynthesis pathway. To better understand the role of DWF1 of monocot plants in BRs biosynthesis, we cloned a maize gene, ZmDWF1, which has extensive sequence similarity to Arabidopsis DWF1. ZmDWF1 is predicted to encode a 562-amino-acid protein that has 95 and 86% similarity with rice OsDWF1 and Arabidopsis DWF1, respectively. Southern blot result showed that one copy of ZmDWF1 is present in maize genome. Northern blot analyses revealed that expression of ZmDWF1 is temporally and spatially regulated during development. Transgenic maize plants with double-stranded ZmDWF1 RNA were generated. Some of them have dwarfed phenotype varying in severity. Western blot results also demonstrated the expected cosuppression of ZmDWF1 gene in transgenic plants.
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