卵黄发生
生物
长角血蜱
滴答声
卵母细胞
血粉
基因敲除
人口
细胞生物学
中肠
基因
内科学
动物
硬蜱科
遗传学
病毒学
生态学
幼虫
社会学
人口学
胚胎
医学
作者
Takeshi Hatta,Naotoshi Tsuji,Takeharu Miyoshi,M. Khyrul Islam,Md. Abdul Alim,Kayoko Yamaji,A Anisuzzaman,Kozo Fujisaki
标识
DOI:10.1016/j.parint.2010.03.001
摘要
Female ixodid ticks are amazing invertebrate animals which efficiently convert a large amount of nutrients derived from their ingested blood meals into eggs. Although oocyte development (vitellogenesis) in ticks is triggered by a blood meal and is assumed to be supported by nutrition derived from ovarian cells connecting oocytes, little is known about the ovarian molecules processing nutrient materials for the vitellogenesis. In this study, we have suggested a putative function of leucine aminopeptidase (HlLAP) in the ovary of parthenogenetic adult ixodid tick Haemaphysalis longicornis regarding a negative output of reproduction following disruption of HlLAP gene by RNA interference. Endogenous HlLAP was shown to be localized in the ovarian cells, including ovarian epithelial and pedicel cells which were assumed to provide nutrients for the developing oocytes. Histological studies demonstrated that a majority of immature oocytes in HlLAP gene knockdown ticks were transformed into abnormal morpho-histological oocytes with vacuolated cytoplasm and/or condensed nucleus. Taken together, a reduction of the numbers of laid eggs in the HlLAP gene knockdown ticks may be due to the degeneration of immature oocytes following deprivation of nutrients such as amino acids supplied not only by midgut HlLAP but also by the ovarian HlLAP. Regulation of the tick molecules involved in nutrient metabolism for the reproduction, including blood digestion and vitellogenesis, would help in controlling the tick population and tick-borne pathogens.
科研通智能强力驱动
Strongly Powered by AbleSci AI