糖组学
计算生物学
生物
有机体
酶
蛋白质组学
功能(生物学)
细胞生物学
生物化学
遗传学
基因
作者
Michelle L. Kovarik,Nancy L. Allbritton
标识
DOI:10.1016/j.tibtech.2011.01.003
摘要
Seemingly identical cells can differ in their biochemical state, function and fate, and this variability plays an increasingly recognized role in organism-level outcomes. Cellular heterogeneity arises in part from variation in enzyme activity, which results from interplay between biological noise and multiple cellular processes. As a result, single-cell assays of enzyme activity, particularly those that measure product formation directly, are crucial. Recent innovations have yielded a range of techniques to obtain these data, including image-, flow- and separation-based assays. Research to date has focused on easy-to-measure glycosylases and clinically-relevant kinases. Expansion of these techniques to a wider range and larger number of enzymes will answer contemporary questions in proteomics and glycomics, specifically with respect to biological noise and cellular heterogeneity.
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