Electrochemical regeneration of nicotinamide cofactor using a polypyrrole rhodium bis-terpyridine modified electrode

A rhodium (III) bis-terpyridine complex covalently linked to two pyrrole groups has been electropolymerized on a reticulated vitreous carbon electrode. The resulting modified electrode has been used in the electrocatalytic reduction of nicotinamide adenine dinucleotide into 1,4-NADH. The efficiency of a system incorporating cyclohexanone as substrate and liver alcohol dehydrogenase as enzyme has demonstrated the feasibility of bioreactors with electrochemical regeneration of NADH cofactor.