HPLC DETECTORS: A BRIEF REVIEW

高效液相色谱法 化学 色谱法 分析化学(期刊)
作者
Michael E. Swartz
出处
期刊:Journal of Liquid Chromatography & Related Technologies [Taylor & Francis]
卷期号:33 (9-12): 1130-1150 被引量:182
标识
DOI:10.1080/10826076.2010.484356
摘要

In an HPLC system the detector is the component responsible for turning a physical or chemical attribute into a measureable signal corresponding to concentration or identity.[ 1 Scott , R.P.W. Chromatographic Detectors. Design, Function and Operation , Marcel Dekker : New York , 1996 .[Crossref] , [Google Scholar] , 2 Snyder , L.R. ; Kirkland , J.J. ; Dolan , J.W. Introduction to Modern Liquid Chroamtography , John Wiley and Sons : New York , 2010 . [Google Scholar] ] In the early days of HPLC, detection was often carried out by collecting fractions and analyzing them off-line. It wasn't until the 1940's and 1950's that the first online detectors for HPLC were introduced.[ 3 Tiselius , A. ; Claesson , D. Arkiv. Kemi Mineral. Geol. 1942 , 15B ( 18 ), 1 . [Google Scholar] , 4 Martin , A.J.P. ; Randall , S.S. Biochem. J. 1951 , 49 , 293 .[Crossref], [PubMed], [Web of Science ®] , [Google Scholar] ] Certainly an improvement over off-line approaches, sensitivity was an issue. The search for more sensitive universal detectors for HPLC led researchers to adapt GC detectors for use in HPLC,[ 5-8 Haati , E. ; Nikkari , T. Acta Chim. Scand. 1963, 17, 2565. Scott , R.P.W. ; Lawrence , J.G.J. Chromatogr. Sci. 1970 , 11 , 65 . Julin , B.G. ; Vandenborn , H.W. ; Kirkland , J.J. J. Chromatogr. 1975 , 112 , 443 . Dolan , J.W. ; Seiber , J.N. Anal. Chem. 1977 , 49 , 326 . ] but the removal of the HPLC mobile phase originally limited applicability. However, in the 1960s the first ultraviolet detector for HPLC was introduced,[ 9 Horvath , C.G. ; Lipsky , S.R. Nature 1966 , 211 , 748 .[Crossref], [PubMed], [Web of Science ®] , [Google Scholar] ] and subsequent improvements in design led to better sensitivity[ 10 Kirkland , J.J. Anal. Chem. 1968 , 40 , 391 .[Crossref], [Web of Science ®] , [Google Scholar] ] and improvements such as variable wavelength and diode array detectors. While a truly universal HPLC detector with GC/FID levels of sensitivity is still elusive, many different types of detectors have been developed that have been very successful for a wide variety of general or specific HPLC applications. To assist in choosing the proper HPLC detector for a given application, this review describes the principles and attributes of many of the common HPLC detectors in use today, and compares and contrasts the advantages and disadvantages of the various detectors discussed where appropriate. For additional detail on the HPLC detectors addressed here, the reader is encouraged to consult the references in each section along with additional general reviews.[ 11 Parriott , D. A Practical; Guide to HPLC Detection , Academic Press : San Diego , CA , 1993 . [Google Scholar] , 12 Scott , R.P.W. Handbook of HPLC , Chapter 15; (Chromaotgraphic Science Series, Vol. 78) , Katz , E. , Eksteen , R. , Schoenmakers , P. , Miller , N. Eds.; Dekker : New York , 1998 . [Google Scholar] ]
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
CipherSage应助aaa采纳,获得10
刚刚
刚刚
珊珊完成签到,获得积分10
6秒前
赘婿应助南楼小阁主采纳,获得10
6秒前
zyyin完成签到,获得积分10
10秒前
kk应助珊珊采纳,获得10
10秒前
12秒前
研友_VZG7GZ应助ll采纳,获得10
12秒前
隐形曼青应助Justtry采纳,获得10
12秒前
13秒前
LongH2完成签到,获得积分10
13秒前
傲安完成签到 ,获得积分10
13秒前
liu发布了新的文献求助10
15秒前
15秒前
大个应助bixiuwu采纳,获得10
16秒前
xxxx完成签到 ,获得积分10
17秒前
量子星尘发布了新的文献求助10
20秒前
21秒前
段晓坤发布了新的文献求助10
21秒前
不想起昵称完成签到 ,获得积分10
22秒前
www123qe发布了新的文献求助10
22秒前
香菜发布了新的文献求助10
23秒前
FFFFFF完成签到 ,获得积分10
23秒前
24秒前
赘婿应助小蘑菇采纳,获得10
24秒前
24秒前
26秒前
26秒前
28秒前
充电宝应助混沌采纳,获得10
28秒前
liu完成签到,获得积分10
30秒前
31秒前
8R60d8应助www123qe采纳,获得10
31秒前
32秒前
科研小辣鸡完成签到,获得积分10
32秒前
32秒前
32秒前
Ammr完成签到 ,获得积分10
33秒前
金鑫水淼发布了新的文献求助10
34秒前
十一发布了新的文献求助10
34秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971644
求助须知:如何正确求助?哪些是违规求助? 3516269
关于积分的说明 11181862
捐赠科研通 3251441
什么是DOI,文献DOI怎么找? 1795889
邀请新用户注册赠送积分活动 876131
科研通“疑难数据库(出版商)”最低求助积分说明 805246