蛋白质组学
色谱法
萃取(化学)
化学
定量蛋白质组学
质谱法
计算生物学
生物
生物化学
基因
作者
Wei Wang,Fuju Tai,Shaoning Chen
标识
DOI:10.1002/jssc.200800087
摘要
Plant tissues usually contain high levels of proteases and secondary metabolites that severely interfere with protein extraction, separation, and identification. Preparation of high-quality protein samples from plant tissues for proteomic analysis represents a great challenge. This article briefly describes the critical points in protein separation, especially secondary metabolites in plant tissues, and removal strategy. It provides an updated overview of three total protein extraction methods and their applications in proteomic analysis of various recalcitrant tissues.
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