纤维二糖
木糖
发酵
阿拉伯糖
戊糖
生物化学
大肠杆菌
化学
生物过程
木质纤维素生物量
木糖代谢
水解
食品科学
生物
纤维素酶
古生物学
基因
作者
Vinuselvi Parisutham,Sung Kuk Lee
标识
DOI:10.1016/j.enzmictec.2011.10.001
摘要
Natural ability to ferment the major sugars (glucose and xylose) of plant biomass is an advantageous feature of Escherichia coli in biofuel production. However, excess glucose completely inhibits xylose utilization in E. coli and decreases yield and productivity of fermentation due to sequential utilization of xylose after glucose. As an approach to overcome this drawback, E. coli MG1655 was engineered for simultaneous glucose (in the form of cellobiose) and xylose utilization by a combination of genetic and evolutionary engineering strategies. The recombinant E. coli was capable of utilizing approximately 6 g/L of cellobiose and 2 g/L of xylose in approximately 36 h, whereas wild-type E. coli was unable to utilize xylose completely in the presence of 6 g/L of glucose even after 75 hours. The engineered strain also co-utilized cellobiose with mannose or galactose; however, it was unable to metabolize cellobiose in the presence of arabinose and glucose. Successful cellobiose and xylose co-fermentation is a vital step for simultaneous saccharification and co-fermentation process and a promising step towards consolidated bioprocessing.
科研通智能强力驱动
Strongly Powered by AbleSci AI